Relationships between Phenotype and Genotype of Escherichia Coli O149:k91, F4 Strains Isolated from Pigs with Diarrhoea
نویسندگان
چکیده
Toxic and fimbrial virulence factors as well as genetic diversity of 11 Escherichia coli O149:K91, F4 strains isolated from pigs with diarrhoea in Germany were examined. The ability of enterobacterial repetitive intergenic consensus – polymerase chain reaction (ERIC-PCR) to discriminate E. coli strains was evaluated. Two different virulence marker profiles: F4/EAST1/LTI/STII and F4/EAST1/LTI/STI/STII were obtained. Comparison of ERIC-PCR fingerprinting revealed a relatively low percentage of genotypic similarity between the tested isolates in spite of the presence almost the same virulence marker genes. The obtained results showed that the ERIC-PCR method could be very useful for study of genetic difference between bacterial isolates especially when the tested strains showed the same virulence markers.
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